PURPOSE: Nucleotide sequence of coat protein gene of CGMMV type Y is useful for the specific diagnosis of viral disease. CONSTITUTION: Nucleotide and amino acid sequences(drawing 3) of coat protein of CGMMV type Y, which are cloned into pGEMT-Easy vector. CGMMV is isolated from cucumber leaves, which are infected with virus and total RNAs of CGMMV are purified by phenol extraction method. The nucleotide sequence of PCR product, which is cloned into pGEMT-Easy vector is determined and amino acid sequence is sequenced therefrom