Shigeo YAGI,Zhijian YANG,Shukuan LI,Xinghua SUN,Yuying TAN
申请号：
US14162655
公开号：
US20140205583A1
申请日:
2014.01.23
申请国别(地区):
US
年份:
2014
代理人:
摘要：
This invention relates to methods of modifying pyridoxal 5′ phosphate (PLP) dependent enzymes to extend the serum half-life of the enzyme, extend the in vivo period of methionine depletion in a host, and decrease the immunogenicity of the enzyme. A preferred PLP-dependent enzyme to be modified is a methioninase, preferably a recombinant methioninase (rMETase). The invention further relates to compositions comprising a modified PLP-dependent enzyme and methods of using the same.
