DE STAAT DER NEDERLANDEN VERT. DOOR DE MINISTER VAN VWS
发明人:
VAN DE WATERBEEMD BAS,VAN DER POL LEONARDUS ALDOLFUS
申请号：
NZ61891812
公开号：
NZ618918A
申请日:
2012.07.05
申请国别(地区):
NZ
年份:
2015
代理人:
摘要：
Discloses a process for detergent-free preparation of bacterial outer membrane vesicles (OMV) for use in vaccines, said process comprising the steps of: a) cultivating a population of a Gram-negative bacterium to stationary growth phase b) at a time point at least about 1 hour after onset of stationary growth phase, incubating the bacteria obtained in a) in a medium adjusted to or having a pH higher than pH 8.0 and a concentration of a metal chelating agent of between about 1 and 100 mM to extract OMV and, c) recovering the OMV extracted in b), wherein the recovery at least comprises removal of the bacteria from the OMV. Also discloses use of an OMV obtained by such a method to elicit immune reactions or treat meningitis.
