This invention relates to a method to producing a spheroid cell population of adult stem cells, comprising culturing adult stem cells on a thin film made by biocompatibility polymer materials. The biocompatibility polymer materials is chitosan, alginate, hyaluronic acid, tremella polysaccharide, polycaprolactone or any combination thereof. The adult stem cells are selected from the group consisting of neural stem cells, neural progenitor cells, adipose-derived adult stem cells, human gingival fibroblasts, bone mesenchymal stem cells, lung stem cell and placenta-derived mesenchymal stem cells. The method can sustain the self-renew and stemness of adult stem cells.本發明係提供一種使成體幹細胞產生為一球體細胞群體的方法,包含體外培養成體幹細胞於一生物相容性高分子形成的一薄膜上,亦可將此薄膜以化學或物理方法被覆於其他基材之表面,其中該生物相容性高分子係為幾丁聚醣、褐藻酸鹽、透明質酸、銀耳多醣、聚己內酯或其任一組合;以及收集由成體幹細胞聚集成之一球體細胞群體,其中該球體細胞群體會擴大且具有自我更新及分化為一體細胞的能力;該成體幹細胞選自由神經幹細胞、神經前驅細胞、脂肪幹細胞、牙齦幹細胞、骨髓間葉幹細胞、肺幹細胞及胎盤幹細胞所組成之群組。另一方面,由成體幹細胞產生的該球體細胞群體進一步具有分化(differentiation)為一心肌細胞或一軟骨細胞或神經相關細胞的能力,此方法可用於篩選純化具較佳自我更新能力的幹細胞,亦可用於製備為一醫藥組合物,而達到細胞自我更新及維持幹性(stemness)的突出效果。
