The present invention relates to a method for preparing a fermented deer antler extract having an anti-inflammatory activity, an extract obtained by the method and to the use of such extract. The method comprises :a step (step 1) of adding purified water to chopped deer antler and performing hot water extraction and filtering on the resultant material a step (step 2) of adding a culture solution for Bacillus sp. strain to the liquid content obtained in step 1 and fermenting the resultant material to obtain a first fermented liquid extract and a step (step 3) of adding purified water to the solid content of deer antler obtained in the first step, adding a culture solution for Aspergiullus sp. strain to the resultant material and fermenting the resultant material to obtain a second fermented liquid extract. According to the present invention, deer antler extracts can be prepared by the most environmentally-friendly fermentation, destruction of physiological active substance of deer antler is reduced while increasing the content of active ingredient, and productivity can be also improved. Therefore, the method of the present invention can be valuably used as a method for preparing deer antler extracts which may be used in various fields. Particularly, the method of the present invention systematically discloses an anti-inflammatory activity, and therefore, can be suitably and widely used in foods, cosmetics, etc.La présente invention concerne un procédé de préparation dun extrait de bois de cerf fermenté ayant une activité anti-inflammatoire, un extrait obtenu par le procédé et lutilisation dun tel extrait. Le procédé comprend : une étape (étape 1) consistant à ajouter de leau purifiée à du bois de cerf broyé et à réaliser une extraction par leau chaude et une filtration sur le matériau résultant une étape (étape 2) consistant à ajouter une solution de culture pour une souche de Bacillus sp. au contenu liquide obtenu à létape 1 et à faire fermenter le matériau résulta
