Recombinetics, Inc.;Regents of the University of Minnesota
发明人:
Asakura, Atsushi,Aravalli, Raj,Cheeran, Maxin,Dutton, James,Garry, Daniel J.,Garry, Mary G.,Graham, Melanie,Li, Ling,Low, Walter,Panoskaltsis-Mortari, Angela,O'Brien, Timothy,Steer, Clifford,Tolar,,OBrien, Timothy,Tolar, J
申请号：
AU2016344152
公开号：
AU2016344152A1
申请日:
2016.10.27
申请国别(地区):
AU
年份:
2018
代理人:
摘要：
Methods for providing human or humanized tissues and organs suitable for transplant are disclosed herein. Gene editing of a host animal provides a niche for complementation of the missing genetic information by donor stem cells. Editing of a host genome to knock out or disrupt genes responsible for the growth and/or differentiation of a target organ and injecting that animal at an embryo stage with donor stem cells to complement the missing genetic information for the growth and development of the organ. The result is a chimeric animal in which the complemented tissue (human/humanized organ) matches the genotype and phenotype of the donor. As disclosed herein, it is possible to do so by simultaneously editing multiple genes in a cell or embryo creating a "niche" for the complemented tissue. Multiple genes can be targeted for editing using targeted nucleases and homology directed repair (HDR) templates in vertebrate cells or embryos.
