MODIFICATION EUKARYOTIC CYTOPLASM DNA VIRUS AND PRODUCTION THEREOF
- 专利权人:
- IMMUNO AG CHEM MED PROD
- 发明人:
- FURIIDORITSUHI DORUNAA,FURIIDORITSUHI SHIEIFURINGAA,FUARUKOOGIYUNTAA FUARUKUNAA,MIKAERU FURAIDERAA
- 申请号：
- JP19920250826
- 公开号：
- JPH06261763(A)
- 申请日:
- 1992.08.26
- 申请国别(地区):
- 日本
- 年份:
- 1994
- 代理人:
- 摘要：
A method is disclosed for producing a modified eukaryotic cytoplasmic DNA virus by direct molecular cloning of a modified DNA molecule comprising a modified cytoplasmic DNA virus genome. The inventive method comprises the steps of (I) modifying under extracellular conditions a DNA molecule comprising a first cytoplasmic DNA virus genome to produce a modified DNA molecule comprising the modified cytoplasmic DNA virus genome; (II) introducing the modified DNA molecule into a first host cell which packages the modified DNA molecule into infectious virions; and (III) recovering from the host cell virions comprised of the modified viral genome. The host cell is infected with a helper virus which is expressed to package the modified viral genome into infectious virions. Examples of packaging a modified poxvirus genome by a helper poxvirus of the same or different genus are described. Also disclosed are novel poxvirus vectors for direct molecular cloning of open reading frames into a restriction enzyme cleavage s
- 来源网站：
- 中国工程科技知识中心
