The invention discloses a method and a system for detection of human physiological status transition. The method includes the steps: initializing parameters, acquiring resistance data of a human body, analyzing data, and reporting detected human physiological status transition conditions to a user, wherein data analysis includes resistance data preprocessing, logarithm taking, window processing, logarithm difference comparison, convergence value calculation and physiological status transition condition analysis. The system comprises a medical pole piece, a bridge resistor/conductivity measuring circuit, an amplifying circuit, an A/D (analog/digital) conversion circuit, a CPU (central processing unit) and a human-computer interaction interface in sequential connection. By means of an adaptive algorithm, different physiological status transition analysis parameters are set up according to different physique conditions of users, and accordingly the problem of individual difference can be solved to reflect physiological status transition of testees more accurately.