CONWAY, ANTHONY,JAIN, SUMITI,LEE, GARY K.,REIK, ANDREAS,TRUONG, LYNN N.
申请号：
CA3071683
公开号：
CA3071683A1
申请日:
2018.08.08
申请国别(地区):
CA
年份:
2019
代理人:
摘要：
Disclosed herein are methods and compositions for engineering cells to expressa CAR where the cells also haveinactivated TCR, HPRT, PD1, CISH and/or HLA genes, using engineered nucleasescomprising at least one DNA binding domain anda cleavage domain or cleavage half-domain in conditions able to preserve cellviability. Polynucleotides encoding nucleases, vectorscomprising polynucleotides encoding nucleases and cells comprisingpolynucleotides encoding nucleases and/or cells comprisingnucleases are also provided.
