The present invention relates to a kind of configurations of polynucleotides for treating the cone cell of disease, such as colour blindness, the carrier of nucleic acid, it includes the polynucleotides, a kind of pharmaceutical composition, carrier comprising the nucleic acid, kit, the carrier comprising the polynucleotides or the nucleic acid, the method for preparing polypeptide, the carrier of the nucleic acid, and the cone cell for the treatment of disease.