M Uthira,K Dheenadhayalan,R subramanian,G Ayyappadasan,P PONMURUGAN
申请号：
IN201641042258
公开号：
IN201641042258A
申请日:
2016.12.10
申请国别(地区):
IN
年份:
2016
代理人:
摘要：
Abstract: A felicitous form of symbiotic relationship formed by a fungus with one or more alga eventually leads to the formation of lichens. The secondary metabolites of lichens provide its source organism, a resistance towards a number of environmental stresses. The properties of lichens are sometimes analogous to plants-but they are not plants. Secondary metabolites play an important role in plant-environment relationship. The secondary metabolites of lichens are originated from the fungal partner-the mycobiont. The unique chemical substances present in lichens, most of which are not a part of other organisms, are classified as depsides, depsidones and dibenzofuran. About 20% of lichen is made up of lichen substances or lichen acids. The compounds are considered very important in defence mechanisms because of the physiological cost in energy and carbon that is spent by the lichens in synthesizing them. Hence, optimising lichen secondary metabolite extraction and purification is a very imprtant research aspect in the phyto- pharmaceutical industry. In order to accomplish this, a standardised column chromatographic method was framed to purify the compounds of the lichen-Rocella montagnei. About 20-50 gms of the lichen was subjected to cold extraction procedure using methanol as a solvent. This was followed by rotary evaporation which yielded 5 g as the crude extract. A preliminary Thin Layer Chromatographic (TLC) procedure was done by using different solvent systems like Hexane : Ethyl acetate and Chloroform : Methanol solvent systems to perform column chromatography. About 23 fractions were collected in Hexane : Ethyl acetate solvent system and 10 were collected from the Chloroform : Methanol solvent system. A perfect crystal was obtained in the fractions 4 to 6 which were eluted in the solvent ratio of 90% Hexane and 10% Ethyl acetate. This was further purified and recrystallized. About 1.755 g of the methyl orsellinate was obtained. Further it was s
