Disclosed is a separator for an electrochemical device including a porous polymer film, and a porous coating layer including at least one type of particles of inorganic particles and organic particles and binder polymer, the porous coating layer formed on one surface or both surfaces of the porous polymer film, wherein the porous polymer film has a structure in which multiple fibrils arranged parallel to the surface of the film are stacked in layers, and the fibrils are bound to each other to form a lamellar structure with a size from 130 to 200 nm and tortuosity of the porous polymer film is from 1.60 to 1.90, and an electrochemical device comprising the same.