The present invention provides a vitrified refrigeration carrier for biological samples, and a fast and simple closed aseptic freezing method for biological samples. Specifically, the carrier includes a main body and a sealing cap, and a proximal end of the main body and a bottom portion of the sealing cap are sealed together to form a sealed frozen sample installation area, and the frozen sample installation area is frozen. When the sample mounting table is provided, and the main body is provided with a frozen liquid circulation passage, and when the biological sample is frozen, the frozen liquid flowing through the passage comes into contact with the outer surface of the frozen sample setting area, In addition, a flow guide hole corresponding to the frozen liquid circulation passage is provided in the sealing cap so as to cool the biological sample in the frozen sample installation region. The vitrification freezing carrier and method of the present invention have the advantages of being safe, non-toxic and highly efficient in vitrification freezing and thawing operations, and have a wide range of potential applications in the field of biological sample freezing.本発明は、生物試料のガラス化冷凍担体、および快速で簡易な生物試料の閉鎖式無菌冷凍方法を提供する。具体的に、前記の担体は、本体と密封キャップとを含み、前記本体の近端と密封キャップの底部が互いに密封して密閉の冷凍試料設置領域を形成し、前記の冷凍試料設置領域に冷凍試料設置台が設けられ、かつ前記の本体に冷凍液体流通通路が設けられ、そして生物試料を冷凍する時、前記通路内を流通する冷凍液体が前記の冷凍試料設置領域の外表面と接触し、かつ冷凍試料設置領域における生物試料を冷却するように、前記密封キャップに前記冷凍液体流通通路に相応する導流孔が設けられる。本発明の生物試料のガラス化冷凍担体および方法は安全で、無毒で、ガラス化冷凍および解凍の操作の効率が高いといった利点があり、生物試料の冷凍の分野で幅広い応用の将来性がある。
