The present invention provides a biological sample vitrification carrier and a method for freezing a biological sample rapidly, simply, conveniently, closely and sterilely. Specifically, the carrier comprises a main body and a sealing cap. A near end of the main body and an inner bottom surface of the sealing cap are mutually sealed to form a sealed frozen sample placing region. The frozen sample placing region is provided with a frozen sample placing platform, the main body is provided with a frozen liquid circulating channel, and the sealing cap is provided with a guide hole corresponding to the frozen liquid circulating channel, so that, when the biological sample is frozen, frozen liquid circulating in the channel contacts with an outer surface of the frozen sample placing region and cools the biological sample in the frozen sample placing region. The biological sample vitrification carrier and the method in the present invention have the advantages of safety, nontoxicity and high vitrification and rewarming operation efficiency and have wide application prospects in the field of biological sample freezing.
