ORGANELLA GENOME-DERIVED SINGLE NUCLEOTIDE POLYMORPHIC PRIMER PAIR FOR TYPE DISCRIMINATION OF ITALIAN RYEGRASS AND DISCRIMINATION METHOD OF ITALIAN RYEGRASS TYPE USING THE PRIMER SET
PROBLEM TO BE SOLVED: To provide a type discrimination method of Italian ryegrass. SOLUTION: An organella genome-derived single nucleotide polymorphic primer pair for type discrimination of Italian ryegrass and a discrimination method of Italian ryegrass type are provided, wherein the type discrimination method of Italian ryegrass includes, by using a primer pair capable of detecting organella genome-derived monobasic polymorphic marker, amplifying the monobasic polymorphic marker by gene amplifying reaction of genome DNA of the test individual, and examining frequency of appearance thereof to determine the type. A rapid, simple and accurate Italian ryegrass type discrimination method using the monobasic polymorphism on genome DNA of organella is provided. COPYRIGHT: (C)2009,JPO&INPIT
