PURPOSE: A plant transformation vector, pCMF, for removing a selective marker is provided to enable easy modification of the vector and transduction of a target gene, and to develop marker-free GM(genetically modified) crops. CONSTITUTION: A site-specific recombinase gene, mFLP, has a base in sequence number 1. A vector for transformation in a plant contains the gene, mFLP. The vector contains a stress-induced promoter and recombinase recognition site. The vector is able to remove a selective marker by induction of mFP expression and self-cleavage due to stress. The vector contains MCS(multi-cloning site). A plant is transformed with the vector. Agrobacterium tumefaciens LBA4404 is transformed by the vector and is used as a plant carrier. A transgenic plant is prepared by transforming with the vector and is able to remove the selective marker.본 발명은 새로운 합성 재조합효소 유전자 및 이를 이용한 선발표지 유전자 제거용 식물형질전환 벡터에 관한 것이다. 본 발명에 따른 선발표지 제거용 식물형질전환 벡터 pCMF는 미생물에서 벡터의 안정성이 증가한 반면 식물체에서의 활성은 유지되며, 목표 유전자의 도입과 벡터의 변형이 용이하여 폭넓은 작물 및 다양한 유전자를 이용한 선발표지 제거(marker-free) GM작물 개발에 이용될 수 있을 것이다.
