The present disclosure relates to the field of healthcare foods. Disclosed is a composition having functions of improving kidney yang, enhancing immunity and relieving fatigue, which is an aqueous extract of ASTRAGALI RADIX, REHMANNIAE RADIX, EUCOMMIAE CORTEX, MORINDAE OFFICINALIS RADIX, LYCII FRUCTUS and POLYGONATI RHIZOMA. By using six traditional Chinese herb medicines, ASTRAGALI RADIX, REHMANNIAE RADIX, EUCOMMIAE CORTEX, MORINDAE OFFICINALIS RADIX, LYCII FRUCTUS and POLYGONATI RHIZOMA for decoction extraction, a composition having functions of improving kidney yang, enhancing immunity and relieving fatigue is obtained. Through synergetic function of each traditional Chinese herb medicine, the composition achieves a better effect comparing with similar products. In addition, the species of traditional Chinese medicine in the composition is relatively fewer, lowering the potential safety hazard, and the method is simple and the cost is low.