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Methods and systems for nucleic acid sequencing by tunneling recognition
专利权人:
Roche Sequencing Solutions, Inc.
发明人:
Astier Yann
申请号:
US201715610186
公开号:
US10190159(B2)
申请日:
2017.05.31
申请国别(地区):
美国
年份:
2019
代理人:
Kilpatrick Townsend and Stockton LLP
摘要:
Embodiments may include a method of analyzing a nucleic acid molecule. The method may include attaching the nucleic acid molecule to a protein. The protein may be attached to a particle with a first diameter. The method may also include applying an electric field to move a first portion of the nucleic acid molecule into an aperture. The aperture may be defined by a first electrode, an insulator, and a second electrode. The aperture may have a second diameter less than the first diameter. The method may further include contacting the first portion of the nucleic acid molecule to both the first electrode and the second electrode. The method may include applying a voltage across the first electrode and the second electrode. The current through the electrodes and the portion of the nucleic acid molecule may be measured, and a nucleotide of the nucleic acid molecule may be identified.
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