Федеральное государственное бюджетное учреждение науки Институт химической биологии и фундаментальной медицины Сибирского отделения Российской академии наук
发明人:
Стеценко Дмитрий Александрович,Купрюшин Максим Сергеевич,Пышный Дмитрий Владимирович
申请号：
RU2014117293
公开号：
RU2014117293A
申请日:
2014.04.29
申请国别(地区):
RU
年份:
2015
代理人:
摘要：
1.Proizvodnye oligonucleotides containing one or more phosphoramidite groups corresponding to general formula (I) :( I) wherein R and the substituents are independently residues of nucleosides or modified nucleosides, including members of the oligonucleotide, or one of them is a residue of a nucleoside or oligonucleotide and the other is a linker to the polymeric support, an organic radical or a hydrogen atom Ryavlyaetsya hydrogen atom H or an organic radikalom.2.Proizvodnoe oligonucleotides according to claim 1, characterized in that the body. esky Rvybiraetsya radical from the group comprising including alkilazidnuyu group, a suitable protective group, -SOR acyl group, an alkoxycarbonyl group -COOR, including 9-fluorenylmethoxycarbonyl group (Fmoc), and -SONRR carbamoyl group, wherein R independently may be the atom hydrogen or an organic radical including protective gruppoy.3. A process for the preparation of oligonucleotides according to Claim. 1, comprising reacting a phosphite (II) with an azide RNpri temperature ranging from 20C to 150C for a period of 1 minute to 24 hours until the conversion of said phosphite (II) to the corresponding oligonucleotide protected derivative of claim . 1, and subsequently removing the protecting groups to yield the unprotected modified oligonucleotide comprising one or more groups phosphoramidite chemistry (I) (II) 4. A method according to claim. 3, characterized in that the isolation and purification is performed resulting modified nucleotide or oligonucleotide by conventional chromatographic or electrophoretic methods of separation and purification of nucleotides or oligonukleotidov.5. A method according to claim. 3 or 4, characterized1.Производные олигонуклеотидов, содержащие одну или более фосфорамидных групп, соответствующих общей формуле (I):(I)где заместители R и Rнезависимо являются остатками нуклеозидов или модифицированных нуклеозидов, в том числе входящих в состав олигонуклеотида, либо один из них является остатком н
