An electrochemical biosensor test strip, comprising: a first insulating substrate having a groove in a widthwise direction; a pair of electrodes parallel in a lengthwise direction on the first insulating substrate; a reagent for reacting with an analyte of interest to generate a current corresponding to the concentration of the analyte, the reagent being fixed in the groove of the first insulating substrate in the manner such as herein described; and a second insulating substrate bonded onto the first insulating substrate, the second insulating substrate forming a capillary space, along with the groove.