CHUNG ANG UNIV INDUSTRY ACADEMY COOPERATION FOUNDATION
发明人:
申请号：
EP14900237.0
公开号：
EP3184639B1
申请日:
2014.30.12
申请国别(地区):
EP
年份:
2019
代理人:
摘要：
The present invention relates to a ribosomal DNA nontranscribed spacer (rDNA NTS) based gene multiple insertion cassette set and a GRAS-grade recombinant yeast strain using the same. Specifically, a gene multiple insertion cassette comprising rDNA NTS fragments and an auxotrophic selective marker having an incomplete promoter is developed, and a safe oral recombinant strain without antibiotic resistant markers is constructed by multiple insertion of an optimum number of gene multiple insertion cassettes into the chromosome of a Saccharomyces cerevisiae strain. Furthermore, the present invention provides a vaccine composition containing, as an active ingredient, the strain, a culture product thereof, a cell lysate, or nodavirus capsid protein (NNV) isolated and purified therefrom. Furthermore, the present invention provides a composition for feed addition, containing, as an active ingredient, the strain, a culture product thereof, a cell lysate, or squalene or oxidosqualene isolated and purified therefrom.
