An ultrasound diagnostic apparatus includes a transmitting and receiving unit that transmits an ultrasound wave to a target object in sequence and receives the ultrasound wave as ultrasound data reflected from the target object including a blood vessel. A first memory unit stores the received ultrasound data. An image generation unit generates an ultrasound image as a sectional image of the blood vessel. A display unit displays the ultrasound image. A region of interest (ROI) setting unit sets a plurality of ROIs on an inner wall and an outer wall of the blood vessel at a designated time. The ROIs are generated from the ultrasound data. A tracing unit traces movement of tissue in the target object corresponding to the ROIs set from the designated time to sequentially following thereafter. A second memory unit stores information of the movement of the tissue for a predetermined duration.