An ultrasound diagnostic apparatus includes a transmitting and receiving unit that transmits an ultrasound wave to a target object and receives the ultrasound wave as ultrasound data reflected from the target object including a long axis direction blood vessel. An image generation unit generates an ultrasound image as a sectional image of the blood vessel. A region of interest (ROI) setting unit sets a first ROI on a vertical straight line at a right angle to the long axis direction and a second ROI on a wall of the blood vessel displayed at a designated time. A tracing unit traces movement of tissue in the target object corresponding to the first and second ROIs from the designated time to sequentially following thereafter by a gradient method using a spatial brightness gradient. A second memory unit stores information of the movement of the tissue for a predetermined duration.