An ultrasound diagnostic apparatus including a transmitting and receiving unit that transmits an ultrasound wave to a target object and receives the ultrasound wave as ultrasound data reflected from a certain region of the target object including a blood vessel, an image generation unit that generates an ultrasound image as a sectional image of the certain region, and a region of interest setting unit that sets a region of interest including a plurality of divided regions in the ultrasound image at a designated time. The region of interest is generated from the stored ultrasound data. The apparatus further includes a tracing unit that traces movement of tissue in the target object corresponding to the divided regions from the designated time to sequentially following thereafter, and a movement measuring unit that measures a movement distance of the tissue at a predetermined time based on the traced movement of the tissue.