UNIVERSITE DE FRANCHE-COMTE;CENTRE HOSPITALIER UNIVERSITAIRE DE BESANCON
发明人:
PHILIPPE HUMBERT,DELPHINE BINDA,CELINE VIENNET-STEINER,SOPHIE ROBIN,GWENAEL ROLIN,HELENE TAUZIN,LIONEL PAZART
申请号：
FR0958919
公开号：
FR2947562B1
申请日:
2009.12.14
申请国别(地区):
FR
年份:
2013
代理人:
摘要：
The invention relates to a method for producing an unshrunken tissue equivalent. Said method is comprising in that: a mixture is produced that contains at least one element of an extracellular matrix； at least one pluridimensional medium is soaked with said mixture； from the components of the mixture, a lattice comprising at least one element of an extracellular matrix is produced, at least at the medium； at least part of the components of said mixture is attached to the structure of the medium； the shrinking of at least the lattice is prevented and at least said lattice is tensioned on said medium； fibroblasts are integrated into the lattice and at least one cell culture of said fibroblasts is carried out, at least in the lattice. The invention also relates to a method for producing a skin equivalent that comprises at least one dermal equivalent formed by an unshrunken tissue equivalent and at least one epidermal equivalent.
