SYSTEMS AND METHODS FOR IMMOBILIZING EXTRACELLULAR MATRIX MATERIAL ON ORGAN ON CHIP, MULTILAYER MICROFLUIDICS MICRODEVICES, AND THREE-DIMENSIONAL CELL CULTURE SYSTEMS
Dongeun Huh,Mark Mondrinos,Cassidy Blundell,Jeongyun Seo
申请号：
US15748066
公开号：
US20180223251A1
申请日:
2016.07.27
申请国别(地区):
US
年份:
2018
代理人:
摘要：
The presently disclosed subject matter provides an approach to address the needs for microscale control in shaping the spatial geometry and microarchitecture of 3D collagen hydrogels. For example, the disclosed subject matter provides for compositions, methods, and systems employing N-sulfosuccinimidyl-6-(4′-azido-2′-nitro-phenylamino)hexanoate (“sulfo-SANPAH”), to prevent detachment of the hydrogel from the anchoring substrate due to cell-mediated contraction.
