STABLE HIGH GINSENOSIDE-YIELDING CALLUS LINE OF PANAX QUINQUEFOLIUM (AMERICAN GINSENG) AND A METHOD FOR DEVELOPING SUCH STABLE HIGH GINSENOSIDE-YIELDING CALLUS LINE OF PANAX QUINQUEFOLIUM
MADER AJA IKUMARU,MADER ARCANA,PALMA ESH,SANGWAN LAZENDER SING,UNIAL GIRISHI CHANDRA
申请号：
KR19990052236
公开号：
KR20010047849(A)
申请日:
1999.11.23
申请国别(地区):
韩国
年份:
2001
代理人:
摘要：
PURPOSE: A stable high ginsenoside-yielding callus line of Panax quinquefolium (American ginseng) and a method for developing such stable high ginsenoside-yielding callus line are provided, thereby the ginsenoside can be mass-produced regardless of the tophographical and climatic conditions. CONSTITUTION: A stable high ginsenoside-yielding callus line of Panax quinquefolium has (a) purple pigmentation, (b) growth index of 204.8 to 246.01 in about 45 days of culture, (c) ginsenoside yield of 0.94 to 1.33% f.wt. in about 45 days of culture, (d) yield of Rb group of ginsenosides of 49 to 55% from the crude extract in about 45 days of culture, and (e) yield of Rg group of ginsenoside of 35 to 49% from the crude extract in about 45 days of culture. A method for the development of a stable Panax callus line from root explants comprises the steps of: (a) establishing the aseptic callus cultures from root explants on basal medium modified after Murashige and Skoog medium to obtain primary callus, (b) transferring the
