An immunogenic composition comprising ofDiphtheriatoxoid antigen (D),tetanustoxoid (T) antigen,Hepatitis Bsurface antigen (HBsAg), inactivated whole-cellB. pertussis(wP) antigen,Haemophilus influenzaetype B (Hib) capsular saccharide conjugated to a carrier protein, Inactivated Polio Virus (IPV) antigen and additionally one or more antigens and the method of preparing the same. A fully liquid combination vaccine, showing improved immunogenicity, reduced reactogenicity and improved stability. Improved methods of formaldehyde inactivation, improved adsorption profile ofDiphtheriatoxoid antigen (D),tetanustoxoid (T) antigen andHepatitis B(HepB) surface antigen adsorbed individually onto aluminium phosphate adjuvant, minimum total aluminum content(Al3+) and optimized concentration of 2-phenoxyethanol (2-PE) as preservative.一種免疫原性組成物，其包含白喉類毒素(Diphtheriatoxoid)(D)抗原、破傷風類毒素(tetanustoxoid)(T)抗原、B型肝炎表面抗原(HBsAg)、去活化之全細胞百日咳博德特氏菌(B. pertussis)(wP)抗原、與載體蛋白軛合之B型流感嗜血桿菌(Haemophilus influenzaetype B)(Hib)莢膜糖、去活化之脊髓灰質炎(Polio)病毒(IPV)抗原和另外之一或多種抗原，及製備彼之方法。一種全液體組合疫苗，其顯示改善之免疫原性、降低之反應原性及改善之安定性。改善之甲醛去活化方法、個別吸附在磷酸鋁佐劑上之白喉類毒素(D)抗原、破傷風類毒素(T)抗原和B型肝炎(HepB)表面抗原之改善的吸附輪廓、最小總鋁含量(Al3+)和作為防腐劑之2-苯氧基乙醇(2-PE)的優化濃度。
