Disclosed herein is a Bacillus strain, Bacillus sp. isolate F727, that produces metabolites with pesticidal activities. Also provided are bioactive compositions and metabolites derived from cultures of Bacillus sp. isolate F727 capable of controlling pests; as well as methods of use of the strain and its metabolites for controlling pests.本發明係提供一種名為芽孢桿菌分離株F727之芽孢桿菌品種,其可產生具有滅殺有害物活性之代謝物。本發明亦提供取自芽孢桿菌分離株F727之生物活性組成物及代謝物,可用於控制有害物。本發明並提供使用該品種及其代謝物以控制有害物之方法。