Provided are bioactive compositions and metabolites derived from Bacillus and particularly Bacillus megaterium cultures responsible for controlling pests as well as their methods of use for controlling pests. Further provided are pesticidal Bacillus megaterium strains.本發明提供取自芽孢桿菌培養物而可用於控制害蟲之生物活性組成物與代謝物,該芽孢桿菌特別是指巨大芽孢桿菌。本發明亦提供該等生物活性組成物與代謝物用於控制害蟲之方法。本發明並提供具有殺蟲性質之巨大芽孢桿菌品種。