Disclosed herein is a glycocojugate vaccine conferring protection against a Gram negative pathogen infection, a method of manufacturing the glycoconjugate vaccine, and use of the glycocojugate vaccine for treating bacterial infection. The glycocojugate vaccine of the present disclosure has the structure of formula (I),wherein, L is a maleimide-type linker, which is connected to the carrier protein via a maleimide bond formed therebetween； and n and m are respectively an integral or a non-integral number between 2 and 20.本文揭露一種醣複合疫苗，其提供能對抗革蘭氏陰性菌感染的保護力、一種製造該醣複合疫苗的方法，以及使用該醣複合疫苗治療細菌感染的用途。本揭露的醣複合疫苗有具化學式(I)的結構：其中L係一馬來醯亞胺型聯結劑，其與載體蛋白透過形成於兩者之間的馬來醯亞胺鍵相連；且n及m分別為介於2至20之間的整數或非整數。
