The present invention provides antisense antiviral compound, composition and use their method and production, the Filoviridae family of the main duplication for inhibiting virus, including Ebola and horse Burger virus. It includes that primate passes through Ebola and Ma Burger viruses that the compounds of this invention, composition and method, which further relate to virus infection in treatment mammal,. Antisense antiviral compound includes that phosphorodiamidate morpholino oligonucleotides (PMOplus) has nuclease-resistant backbones, the base of about 15-40 nucleotide, at least two, but usually not more than more than half piperazines contain subunit linkage, it is target and initiation site Ebola virus AUG region VP35, Ebola virus VP24, Marburg virus VP 24 with targeting sequence, or Marburg virus N P, including a combination thereof and mixture.
