METHOD FOR SEPARATING X-CHROMOSOME-CARRYING SPERMATIC CELLS AND Y-CHROMOSOME-CARRYING SPERMATIC CELLS
- 专利权人:
- KSI, INK.
- 发明人:
- EHVANS KENNET M.,VAN MUNSTER EHRIK B.
- 申请号：
- RU20020132899
- 公开号：
- RU2297198(C2)
- 申请日:
- 2001.05.09
- 申请国别(地区):
- 俄罗斯
- 年份:
- 2007
- 代理人:
- 摘要：
FIELD: medicine, artificial insemination. ^ SUBSTANCE: the innovation consists of the following stages: selection of spermatic cells from a male of a certain mammalian type except a human being; introduction of spermatic cells into the flow of fluid medium; analysis of spermatic cells taken by the flow of fluid medium; detection of DNA quantity in the nuclei of spermatic cells; irradiation of dyed DNA in the nuclei of spermatic cells; detecting the fluorescent light transmitted by irradiated dyed DNA being in the nuclei of spermatic cells; differentiation of spermatic cells based upon the quantity of DNA being inside the nuclei of spermatic cells located in the present drop; electrostatic deviation of every drop; separate accumulation of drops based upon the quantity of DNA inside the nuclei of spermatic cells captured into each drop; separation of differentiated spermatic cells into X-chromosome-carrying populations and Y-chromosome-carrying ones; and obtaining the populations of intact X-chromosome-carry
- 来源网站：
- 中国工程科技知识中心
