A biometric detection module including a light source module, a detection region and a control module is provided. The light source module is configured to emit green light, red light and IR light in a time division manner to illuminate a skin surface. The detection region is configured to detect penetration light emitted from the light source module for illuminating the skin surface and passing through body tissues to correspondingly generate a green light signal, a red light signal and an IR light signal. The control module is configured to determine a filtering parameter according to the green light signal to accordingly filter the red light signal and the IR light signal, and calculate a biometric characteristic according to at least one of the green light signal, a filtered red light signal and a filtered IR light signal.