System of the present invention is in relation to a kind of thinleaf glossogyne herb extract and application thereof and preparation method, the preparation method is mainly gradually to prepare thinleaf glossogyne herb via separate modes such as solvent extraction, col-umn chromatography, efficient col-umn chromatographies to can inhibit or the compound that prevents lung cancer cell growth and combinations thereof person； In addition, the present invention also discloses the application method of related anti-lung cancer substance. 本發明係有關一種香茹草萃取物及其用途與製備方法，該製備方法主要係將香茹草經由溶劑萃取、管柱層析、高效管柱層析等分離方式，逐步製備出可抑制或預防肺癌細胞生長的化合物及其組合物者；此外，本發明亦揭示相關抗肺癌物質之應用方法。
