A method for extracting antioxidant substances from the tender leaves of a lotus fruit tree, which includes crushing the tender leaves of a lotus fruit tree to form a fragmented young leaf; soaking the fragmented young leaves in alcohol for a predetermined time to form a mixture; filtering the mixture The filtrate is obtained; the filtrate is dried to obtain a dried substance; the dissolved substance containing ethyl acetate and n-hexane is used to dissolve the dried substance and mixed with silicone powder to form a first solid component; The liquid extract performs first silica gel column chromatography on the first solid component, and collects the collected liquid through the first silica gel column chromatography.一種由蓮霧果樹的嫩葉中提取抗氧化物質的方法,包含對蓮霧果樹的嫩葉進行碎化處理,形成碎化的嫩葉;浸泡碎化的嫩葉於醇中一段預定時間,形成混合物;過濾混合物而獲得濾液;乾燥濾液而獲得乾燥物;利用包含乙酸乙酯及正己烷的溶解組分溶解乾燥物,並與矽膠粉混合形成第一固態狀組分;利用包含乙酸乙酯及正己烷的沖提液對第一固態狀組分進行第一矽膠管柱層析處理,並收集通過第一矽膠管柱層析處理的收集液。