A vaccine composition and method which is effective in preventing or ameliorating Avian Influenza Virus infection is set forth herein. The vaccine contains at least two inactivated strains of avian influenza virus, wherein the combined haemoagglutinin (HA) total is at least about 200 HA/dose of the vaccine composition, and wherein each of the strains presents at least about 128 HA/dose, and further wherein one of the strains has the same HA subtype as that of a challenge virus, and wherein at least one of the strains has a different NA subtype than the challenge virus.本文陳述一種有效預防或改善禽流感病毒感染之疫苗組成物及方法，此疫苗含有至少2株不活化的禽流感病毒，其中合併之血球凝集素(HA)總量為該疫苗組成物之至少約200HA/劑，且其中該病毒株每一者呈現至少約128HA/劑，又其中該株之一者具有相同於挑釁病毒之HA亞型，及其中該株之至少一者具有不同於挑釁病毒之NA亞型。
