PURPOSE: A cosmetic composition containing a mixture extract is provided to suppress iNOS and to ensure antibacterial and antioxidative functions.CONSTITUTION: A cosmetic composition contains a mixture extract of Capsosiphon fulvescens, Enteromorpha prolifera, and Sargassum fulvellum. The cosmetic composition is used for antibacterial, antioxidative, and anti-inflammatory effects. A method for preparing the mixture extract comprises: a step of drying and pulverizing Capsosiphon fulvescens, Enteromorpha prolifera, and Sargassum fulvellum in 10-200 mesh a step of adding 80%(v/v) ethanol solution and performing sonication to extract active ingredients in a cell membrane a step of maceration at room temperature for 24 hours a step of filtering using 0.40 um filter and a step of concentrating the extract at 45 Deg. C.COPYRIGHT KIPO 2013[Reference numerals] (AA) DPPH removal ability본 발명은 피부 진정 및 피부자극완화 효과를 가지는 화장료 조성물에 관한 것으로, 더욱 상세하게는 주요 구성 해조추출물로서 매생이, 파래 및 모자반 추출물을 혼합하여 민감성 피부를 보호하여 피부 진정 효과 및 피부자극완화 효과를 가지는 우수한 화장료 조성물을 제공하는 것이다.