COSISBIO CO., LTD.;KOREA NATIONAL UNIVERSITY OF TRANSPORTATION INDUSTRY-ACADEMIC COOPERATION FOUNDATION;COSIS CO., LTD.
发明人:
KIM, DONG GOO,김동구,PAIK, CHUN KYU,박천규,SHIN, JI YOUNG,신지영,CHANG, LAE SUNG,장래성,JUNG, JAE HYUN,정재현,YU, KWANG WON,유광원,KIM, HOON,김훈
申请号：
KR1020110079162
公开号：
KR1012234640000B1
申请日:
2011.08.09
申请国别(地区):
KR
年份:
2013
代理人:
摘要：
PURPOSE: A method of culturing mushroom is provided to culture mushrooms containing beneficial components of Hippophae rhamnoides by cultivating the mushrooms in a culture medium containing powder of the Hippophae rhamnoides. CONSTITUTION: A mycelium is obtained by inoculating spawns of lion`s mane mushrooms into a plating medium, and cultivating for 13 to 17 days at the culture temperature of 23 deg. C to 26 deg. C(S110). Multiple mycelium discs are manufactured by cutting the mycelium(S120). The first inoculum source is obtained by inoculating a mycelium disc into a MCM medium and shake-cultivating for 7-12 days at the culture temperature of 23deg. C to 26deg. C(S130). The second inoculum source is obtained by inoculating the first inoculum source into the MCM medium and shake cultivating for 5-7 days at the culture temperature of 23deg. C to 26deg. C(S140). After inoculating the second inoculum source into the MCM medium containing powder of Hippophae rhamnoides, the MCM medium is shake cultivated for 8-10 days at the culture temperature of 23deg. C to 26deg. C(S150). The volume of the first inoculum source to be inoculated into the MCM medium in the stage of obtaining the second inoculum source is 10 vol% to 15 vol% of the total volume of the first inoculum source obtained in the stage of obtaining the first inoculum source. The volume of the second inoculum source to be inoculated into MCM medium in the stage of shake cultivation of the second inoculum source is 10 vol% to 15 vol% of the total volume of the second inoculum source obtained in the stage of obtaining the second inoculum source. [Reference numerals] (AA) Start； (BB) End； (S110) Inoculating spawns of lion`s mane mushrooms into a plating medium, and cultivating for 13-17 days at 23-26 deg. C； (S120) Manufacturing multiple mycelium discs by cutting the mycelium； (S130) Obtaining a first inoculum source by inoculating the mycelium discs in an MCM medium and shake-cultivating for 7-12 days at 23-26 deg.
