The present invention relates to a newly identified bio-surfactant producing bacterial strain, Bacillus subtilis QVS1, which was deposited under accession number LMG P-30406, and to fermentative processes wherein such a strain is employed for surfactin and fengycin (cyclic lipopeptides)-containing biosurfactant production, to crude biosurfactant mixtures or preparations obtained by such processes, and to advantageous culture media comprising red beans and culture conditions for bio-surfactant producing micro-organisms.