Andreas Anton;Daniela Kathmann;Antje Parthier;Susan Lorey;Bernhard Janowski;Heiko Pultke
发明人:
Susan Lorey,Bernhard Janowski,Heiko Pultke,Daniela Kathmann,Antje Parthier,Andreas Anton
申请号：
US14366460
公开号：
US09617322B2
申请日:
2012.12.19
申请国别(地区):
US
年份:
2017
代理人:
摘要：
The present invention relates to a proNGF mutant and to uses thereof, in particular the use of a proNGF mutant for producing human beta-NGF. The present invention discloses a method of preparing a biologically active human beta-NGF from an inactive insoluble proNGF mutant. A proNGF mutant of the invention is substituted by any amino acid but not Arg or Lys at the native protease cleavage site R1SK3R4 at least at positions R1 and K3 corresponding to positions 101 and 103 of the human wildtype proNGF sequence.
