LOREY, SUSAN S,JANOWSKI, BERNHARD B,PULTKE, HEIKO H,KATHMANN, DANIELA D,PARTHIER, ANTJE A,ANTON, ANDREAS A,‧洛里,‧簡諾瓦斯基,‧帕特克,‧卡特曼,‧帕蒂爾,‧安東
申请号：
HK15102539.7
公开号：
HK1202055A
申请日:
2015.03.12
申请国别(地区):
HK
年份:
2015
代理人:
摘要：
The present invention relates to a proNGF mutant and to uses thereof, in particular the use of a proNGF mutant for producing human beta-NGF. The present invention discloses a method of preparing a biologically active human beta-NGF from an inactive insoluble proNGF mutant. A proNGF mutant of the invention is substituted by any amino acid but not Arg or Lys at the native protease cleavage site R 1 SK 3 R 4 at least at positions R 1 and K 3 corresponding to positions 101 and 103 of the human wildtype proNGF sequence.
