SUSAN LOREY,BERNHARD JANOWSKI,HEIKO PULTKE,DANIELA KATHMANN,ANTJE PARTHIER,ANDREAS ANTON
申请号：
PT12805695
公开号：
PT2672984E
申请日:
2012.12.19
申请国别(地区):
PT
年份:
2015
代理人:
摘要：
The present invention relates to a proNGF mutant and to uses thereof, in particular the use of a proNGF mutant for producing human beta-NGF. The present invention discloses a method of preparing a biologically active human beta-NGF from an inactive insoluble proNGF mutant. A proNGF mutant of the invention is substituted by any amino acid but not Arg or Lys at the native protease cleavage site R 1 SK 3 R 4 at least at positions R 1 and K 3 corresponding to positions 101 and 103 of the human wildtype proNGF sequence.
