The invention relates to an anti-bacteria and anti-insect detergent and a manufacturing method thereof. The anti-bacteria and anti-insect detergent comprises a raw material including melia azedarach oil and an emulsifying agent. The detergent with anti-bacteria and anti-insect effects can be formed by the steps of: firstly treating the melia azedarach oil and the emulsifying agent in a pre-dissolution process, which is then treated in a mixing and stirring process with water, and finally treated with a second stirring process and a stationary process. Further, the raw material of the anti-bacteria and anti-insect detergent can be added with citronella essential oil and citrus essential oil, thereby increasing effects of repelling harmful insects and providing pleasant fragrances with pure, fresh and comfort.一種抑菌防蟲清潔劑及其製作方法,該抑菌防蟲清潔劑之原料係為一苦楝油及一乳化劑,而該苦楝油及該乳化劑必須先進行預溶製程,再與水進行混合攪拌製程,最後再進行二次攪拌及靜置製程,即可形成一具有抑菌防蟲功效之清潔劑;另外該抑菌防蟲清潔劑之原料亦可添加一香茅精油及一柑橘精油,除了能夠增加驅除害蟲之功效之外,亦能使該抑菌防蟲清潔劑具有令人清新舒爽之芳香。