INVIRAGEN, INC.; AS REPRESENTED BY THE SECRETARY;THE GOVERNMENT OF THE UNITED STATES OF AMERICA; DEPARTMENT OF HEALTH AND HUMAN SERVICES;THE GOVERNMENT OF THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES
发明人:
DAN T. STINCHCOMB,CLAIRE Y. HUANG,RICHARD M. KINNEY,JILL AL. LIVENGOOD
申请号：
ARP140101223
公开号：
AR095598A1
申请日:
2014.03.17
申请国别(地区):
AR
年份:
2015
代理人:
摘要：
The Methods in this paper Report on compositions, Applications and Manufacture of constructions of Dengue Virus and Live Attenuated dengue. Some embodiments relate to a composition that includes, but is not limited to, a composition tetravalent dengue virus.In some ways, the compositions may include one or more structures of Dengue Virus serotypes, such as construction of Dengue Virus (DEN) - 1 - 1 - 2, dengue virus (den 2), dengue virus (DEN) - 3 or - 4 dengue virus (den - 4).In other modes, The constructions presented in this paper can be combined in a composition to create a Vaccine against one or more structures of dengue virus which may or may not be passed later in Mammalian Cells.Claim 1: a Chimeric Nucleic Acid comprising a First Nucleotide sequence encoding the non Structural Proteins of a virus strain S3 PDK modified Live Attenuated dengue - 2 and a Second sequence of nucleotides that encode at least one minibudgets Protein Ctural dengue 1, where the live virusAttenuated dengue - 2 also comprises at least one additional mutation is a mutation at position 3823 Nucleic Acid, where the position of the amino acid nsza 116 Gene is a Leucine instead of isoleucine.Las modalidades en el presente documento informan sobre composiciones, aplicaciones y fabricación de construcciones de virus del dengue y de virus vivos atenuados del dengue. Algunas modalidades se refieren a una composición que incluye, pero no se limita a, una composición tetravalente del virus del dengue. En ciertas modalidades, las composiciones pueden incluir construcciones de uno o más serotipos del virus del dengue, tales como construcciones del virus del dengue-1 (DEN-1), virus del dengue-2 (DEN-2), virus del dengue-3 (DEN-3) o virus del dengue-4 (DEN-4). En otras modalidades, las construcciones divulgadas en el presente documento pueden combinarse en una composición para generar una vacuna contra una o más construcciones del virus del dengue que pueden o no ser pasadas posteriormente en células
