NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE& TECHNOLOGY
发明人:
申请号：
JP20100107003
公开号：
JP2011234642(A)
申请日:
2010.05.07
申请国别(地区):
日本
年份:
2011
代理人:
摘要：
PROBLEM TO BE SOLVED: To provide a method for efficiently introducing foreign genes into bird primordial germ cells as a method for creating transgenic birds to establish a stable expression strain. SOLUTION: The method for introducing genes into bird primordial germ cells includes following steps 1 and 2: (1) a step of fractionating primordial germ cells derived from birds by a density gradient centrifugation method using a culture medium and sol as a density gradient liquid; and (2) a step of collecting the cells fractionated in the step 1 and introducing genes into the cells. COPYRIGHT: (C)2012,JPO&INPIT
