SEBO, Lubomir,SHEN, Gene,YUE, Stephen,OSUNA, Honey,LAPIN, Yuri,BROGLEY, Louis,FEDOROV, Andrei
申请号：
EP16867352
公开号：
EP3376997A4
申请日:
2016.21.11
申请国别(地区):
EP
年份:
2019
代理人:
摘要：
There is provided label nucleotide isoreagent its include at least: an affinity prime, at least one dyestuff-labeled compound and at least one nucleotide compound. Isoreagent is included in high density, such as the analysis of light reaction that is highly multiplexed in large quantities of individual molecule real-time nucleic acid serial response is useful to various fluorescence-based analysis methods. The high sensitivity that isoreagent is used in desirable wavelength is detectable. They include that transformations of that isoreagent with DNA polymerases of structure member interact, thus the light injury successively decreased and the movement in turn improving isoreagent and other attributes react. Additionally provide the compound of the dye marker of nucleotide and main body isoreagent, and the preparation useful intermediates to compound and isoreagent. Composition includes compound, the synthesis of intermediate, compound and isoreagent, additionally provides mutant DNA polymerases.
