A plasma generator, a surface treatment method using the same, and a surface treatment method using the same for bio-tissue are provided. The plasma generator comprises a plasma tube, a reaction source tube, a first electrode and a second electrode. The plasma tube has a plasma outlet. The reaction source tube is disposed within the plasma tube and has a reaction outlet. The first electrode and the second electrode are disposed the plasma tube, wherein the second electrode is closer to the plasma outlet than the first electrode is. The outlet of the reaction source tube is not projected beyond a lower portion of the first electrode.一種電漿產生裝置、應用其之表面處理方法與生物組織表面處理方法。電漿產生裝置包括電漿導管、反應源導管、第一電極及第二電極。電漿導管具有電漿出口。反應源導管設於電漿導管內,且具有反應物出口。第一電極及第二電極設於電漿導管上,其中第二電極比第一電極鄰近於電漿出口。反應源導管的反應物出口不突出超過第一電極之下緣。100‧‧‧電漿產生裝置110‧‧‧電漿導管110a‧‧‧電漿出口120‧‧‧電漿氣體源130‧‧‧反應源導管130a‧‧‧反應物出口140‧‧‧反應物源150‧‧‧第一導電片160‧‧‧第二導電片170‧‧‧電源供應單元180‧‧‧第一電極180b‧‧‧下表面180u‧‧‧上表面181‧‧‧第一突出部190‧‧‧第二電極191‧‧‧第二突出部G1‧‧‧電漿源氣體G2‧‧‧反應物H‧‧‧間隔P‧‧‧電漿S‧‧‧表面處理元素
