MATERIALS AND METHODS FOR CHARACTERIZING AND USING A 3-KETOACYL-ACYL CARRIER PROTEIN (ACP) SYNTHASE III (KASIII) FOR PRODUCTION OF BI-FUNCTIONAL FATTY ACIDS
GARG Shivani,JIN Huanan,YANDEAU-NELSON Marna,NIKOLAU Basil J.
申请号：
US201715721514
公开号：
US2018051263(A1)
申请日:
2017.09.29
申请国别(地区):
美国
年份:
2018
代理人:
摘要：
A mutant Bacillus subtilis, which does not express a functional KASIIIA and/or KASIIIB, and method of making; a mutant Rhodospirillum rubrum, which does not express a functional PhaC1, PhaC2, and/or PhaC3, and method of making; method of characterizing substrate specificity of KASIII; method of making mutant KASIII with altered substrate specificity and/or altered level of activity and nucleic acid, vector, host cell/organism, and mutant KASIII; an in vitro, high-throughput spectrophotometric method of assaying KASIII activity; and materials and methods for using KASIII for production of bi-functional fatty acids and the materials so produced.
