It is intended to provide supports for hybridization useful in the fields of molecular biology, biochemistry and the like whereby DNA can be efficiently clarified without injuring the terminal parts of the DNA. A support having an oligonucleotide, a cDNA or a gDNA immobilized thereon which is produced by chemically modifying a support, immobilizing the oligonucleotide, cDNA or gDNA hybridized therewith and then dehybridizing it to thereby give a support for immobilizing a nucleotide having an oligonucleotide boded thereto.